Nanoparticle-mediated methods for antimicrobial susceptibility testing of bacteria

摘要

A method of testing bacterial cells for antimicrobial susceptibility includes preparing a suspension of the bacterial cells in a non-nutrient medium, mixing with the suspension an antimicrobial, a carbohydrate usable by the bacterial cells, metallic nanoparticles, and a lectin, and incubating the mixture while monitoring a parameter of the nanoparticles responsive to use of the carbohydrate by the bacterial cells. More broadly stated, the invention includes a method of testing an agent for its effect on cell metabolism by preparing a suspension of cells in a non-nutrient medium, mixing the suspension with the agent, adding a carbohydrate usable by the cells, metallic nanoparticles, and a lectin with binding specificity for the added carbohydrate, and monitoring a nanoparticle parameter responsive to the cells.

主权项

1. A method of testing bacterial cells for antimicrobial susceptibility, the method comprising:
mixing a suspension of bacterial cells in a non-nutrient medium with an antimicrobial and a carbohydrate usable by the bacterial cells; adding iron oxide nanoparticles and a lectin to the mixture; and monitoring a parameter associated with the nanoparticles responsive to use of the carbohydrate by the bacterial cells.