Method of identifying an agent for inhibiting odor of pyrazine derivatives

摘要

A method to identify an agent for reducing an odor of a pyrazine derivative, comprising:
adding a test substance and a pyrazine derivative represented by the following Formula (I):;
wherein R1 represents methyl, ethyl, or acetyl; and R2, R3, and R4 each independently represent hydrogen or methyl, to an olfactory receptor OR5K1 or a polypeptide having at least 80% identity to the amino acid sequence of the olfactory receptor OR5K1;
measuring a response of the olfactory receptor to the pyrazine derivative; andidentifying a test substance inhibiting the response of the olfactory receptor on the basis of the measured response.

主权项

1. A method to identify an agent for reducing an odor of a pyrazine derivative, comprising:
adding a test substance and a pyrazine derivative represented by the following Formula (I):wherein R1 represents methyl, ethyl, or acetyl; and R2, R3, and R4 each independently represent hydrogen or methyl, to a recombinant cell genetically engineered to express an olfactory receptor OR5K1 or a polypeptide which maintains responsiveness to a pyrazine derivative and which has at least 90% identity to the amino acid sequence of SEQ ID NO:2, in a cultured cell;
measuring a response of the olfactory receptor to the pyrazine derivative; and by comparing the response of the olfactory receptor or the polypeptide in the presence of the test substance to the response of the olfactory receptor or the polypeptide to the pyrazine derivative in the absence of the test substance, thereby identifying a test substance inhibiting the response of the olfactory receptor or the polypeptides on the basis of the measured response.