Method and kit for testing carbamates in amine-cured epoxy materials

摘要

The present invention provides a kit and a method of testing for carbamates in amine cured epoxies. The method and kit are based on the use of an enzyme that is inhibited by carbamates. A specific embodiment of the kit includes acetylthiocholine (ATC), dithio-bis (2-nitrobenzoic acid) (DTNB), and acetyl-cholinesterase (ACh-E). In the corresponding method embodiment, the method comprises the steps of collecting carbamates from the epoxy, adding the collected carbamates to a solution, adding acetyl-cholinesterase (ACh-E) to the solution, adding acetylthiocholine (ATC) to the solution, adding dithio-bis (2-nitrobenzoic acid) (DTNB) to the solution, and ultimately measuring the intensity of the yellow color of the solution.

主权项

1. A method for detecting the presence of amine blush in an amine-cured epoxy material, said method comprising the steps of:
a. Collecting, in an aqueous solution, carbamates from said material, said aqueous solution being a test sample, said aqueous solution being a solution comprising water; b. Adding an enzyme whose activity is inhibited by carbamates to the test sample; c. Adding a substrate for said enzyme to the test sample such that cleavage of the substrate by the enzyme will result in either a modified substrate or a substrate cleavage product; d. Adding a chromogen to the test sample, such that said chromogen reacts with either the modified substrate or the substrate cleavage product so as to alter or intensify the color of the test sample; e. Subsequent to the foregoing steps (a), (b), (c), and (d) determining the intensity of the color of the test sample; f. Wherein the smaller the intensity of the color in the test sample, the greater the probability that the test sample suffers from amine blush.