Isolated bioactive compounds and method of use

摘要

Bioactive compounds extracted from a fermentation broth created by a unique microbial community during a fermentation process and fractionated into several fractions based on size. Four of the resulting fractions stimulate growth of plants that are planted in soil to which the fractions have been applied. The fractions can be applied to the soil individually or combined together prior to application. In another embodiment, the unique fractions can be freeze dried or spray dried.

主权项

1. A method of isolating bioactive compounds comprising:
apportioning an amount of a fermentation extract solution; removing biological matter resulting in a clarified liquid; mixing the clarified liquid with an ammonium sulfate solution resulting in a precipitate; isolating the precipitate by centrifugation; redissolving the precipitate in a phosphate buffer; fractionizing the redissolved precipitate into seven fractions, each fraction sequentially decreasing in compound size; removing a set of one or more chosen fractions of the seven fractions; and, applying the set of one or more chosen fractions to a portion of soil media adjacent a plant.