GRADIENT MICROFLUIDIC CHIP DEVICE

摘要

According to an embodiment of the present invention, a concentration gradient microfluidics chip device is provided to identify an inflammation change of nucleus pulposus and annulus fibrosus according to a concentration change of macrophage migration inhibitory factor and annulus fibrosus change, etc. when a neuron is stimulated in real time by generating a plurality of similar intervertebral disc structures to that of a human body on one culture plate and generating the similar intervertebral disc structures on the same plane. The device according to an embodiment of the invention comprises: a culture plate in which a nucleus pulposus chamber having nucleus pulposus cultured therein, an annulus fibrosus chamber having annulus fibrosus cultured therein, and a neuron chamber having a neuron cultured therein are arranged on the same plane from one side direction to an opposite side direction sequentially; an inlet unit in which a first inlet hole having the macrophage migration inhibitory factor inputted therein and a second inlet hole having a buffer solution inputted therein are separated from each other on one side of the culture plate, and connected to the nucleus pulposus chamber individually; a first channel unit through which the nucleus pulposus chamber and the annulus fibrosus chamber are connected to each other and the nucleus pulposus and the annulus fibrosus are contacted to each other physically; and a second channel unit through which the nucleus pulposus chamber and the neuron chamber are connected to each other.