TECHNOLOGY FOR CELL TRANSDIFFERENTIATION

摘要

This invention provides technology for transdifferentiating cells from one cell type to another. The cells are cultured with one or more vector-free gene regulator oligonucleotides concurrently or in succession, and then harvested when cell markers or the morphology of the culture shows that transdifferentiation is complete. Suitable gene regular oligonucleotides include microRNAs and messenger RNAs that encode a differentiation factor. Conditions for transdifferentiation can be optimized by dividing cells into different culture chambers of a microfluidic device. Cells are cultured with different additives in each chamber, and then compared. Transdifferentiated cells produced according to this invention can provide a consistent source of tissue for use in regenerative medicine.

主权项

1. A method for transdifferentiating a cell from a first cell type to a second cell type, comprising culturing the cell in medium containing an oligonucleotide composition comprising one or more vector-free gene regulator oligonucleotides so that the cell is transdifferentiated from the first cell type to the second cell type.