| 摘要 |
The present invention pertains to fields of biochemistry and molecular biology, relates to an αO-superfamily conotoxin peptide, pharmaceutical composition thereof, preparation method and use thereof. The present invention further relates to a propeptide of the conotoxin peptide, nucleic acid construct thereof, expression vector and transformed cell thereof, and fusion protein thereof. The present invention further relates to a method for blocking acetylcholine receptors as well as a use of the conotoxin peptide in the manufacture of a medicament. The new αO-superfamily conotoxin peptide of the present invention is capable of specifically blocking acetylcholine receptor (nAChRs) (e.g., α9α10 nAChR), and NMDA receptor (e.g., NR2C NMDAR), and has activity for treatment of neuralgia, addiction, and activity for treatment of chemotherapy of cancers, breast cancer, lung cancer, wound healing, epilepsia, ischemia, and thus is promising in the manufacture of analgesic, a medicament for treatment of addiction, and a tool drug for neuroscience. |
| 主权项 |
1. A polypeptide, which is or comprises one or more same or different amino acid sequences selected from any one of the following items (1) to (3):
(1) an amino acid sequence as shown in any one of sequences of SEQ ID NO: 7-12; (2) an amino acid sequence having at least 80%, preferably at least 85%, more preferably at least 90%, especially preferably at least 95%, most preferably at least 97% identity with the amino acid sequence of (1); or (3) an amino acid sequence different from the sequence of (1) in substitution, deletion, insertion and/or addition of 1-5, preferably 1-3, more preferably 1-2, most preferably 1 amino acid residue. |
