| 摘要 |
Perioperative patient blood glucose concentrations are determined by imposing patient effluent ultrafiltrate through a sample cell incorporated in an automated polarimeter. The device includes an optical platform, fluid handling subassembly, controlling electronics, and integration software. A stable collimated light source of known intensity and distinct specified wavelength is passed through an optical platform including a polarizer, retarder, bandpass filters, sample flow cell, analyzer and detector. The angular rotation of the transmitted light resulting from the glucose contained in patient ultrafiltrate collected in the sample flow cell is recorded and provides a measure of the glucose concentration. |
| 主权项 |
1. Apparatus for measuring the concentration of a chiral analyte in a sample comprising:
(a) an optical platform having;
i. a light source of monochromatic light,ii. a first polarizer positioned to receive monochromatic light from said light source and having a direction of polarization,iii. a retarder positioned to receive polarized light from said first polarizer,iv. a calibration cell positioned to receive light from said retarder,v. a measurement cell for selectively receiving a flush solution, distilled water, or a solution whose concentration of chiral analytes are to be measured, said measurement cell positioned to receive polarized light from said retarder,vi. an analyzer positioned to receive light from said measurement cell and having a direction of polarization perpendicular to the polarization of said first polarizer, andvii. a detector for receiving light from said analyzer and generating an electrical signal proportionate to the light striking the detector,whereby light from said light source is polarized in said first polarizer and passes through said retarder, calibration cell, measurement cell, and analyzer and strikes said detector to produce an electrical signal proportional to the amount of light received from said analyzer, and
(b) a fluid flow system having:
i. an ultrafilter for receiving fluid having a concentration of chiral analytes to be measured, the output of which yields an ultrafiltrate, andii. means connecting said ultrafilter to said measurement cell for delivering said ultrafiltrate to said measurement cell,whereby light from said light source is polarized and directed through said measurement cell containing said ultrafiltrate, through said analyzer to said detector to generate an electrical signal whose magnitude is a representation of the degree of rotation of polarized light resulting from the polarized light passage through said ultrafiltrate in said measurement cell. |
