发明名称 |
METHOD FOR HIGHLY EFFICIENT CONVERSION OF HUMAN STEM CELLS TO LINEAGE-SPECIFIC NEURONS |
摘要 |
The present invention relates to the field of stem cells. More specifically, the present invention provides methods and compositions useful for the highly efficient conversion of human stem cells to lineage-specific neurons. In a specific embodiment, a method of inducing differentiation of human stem cells into dopaminergic (DA) neurons comprises the steps of (a) transfecting human stem cells with a lentiviral vector encoding Atoh1, wherein the vector is Dox inducible; and (b) growing the transfected cells in culture in the presence of Dox, Sonic Hedgehog (SHH) and FGF-8b until DA neurons are induced. |
申请公布号 |
WO2015084908(A1) |
申请公布日期 |
2015.06.11 |
申请号 |
WO2014US68273 |
申请日期 |
2014.12.03 |
申请人 |
THE JOHNS HOPKINS UNIVERSITY;KENNEDY KRIEGER INSTITUTE, INC. |
发明人 |
YING, MINGYAO;LATERRA, JOHN |
分类号 |
C12N5/079;A61K35/30;A61P25/00;C12N5/0793;C12N15/86 |
主分类号 |
C12N5/079 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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