DEVICE AND METHOD FOR PERFORMING DIGITAL PCR

摘要

PROBLEM TO BE SOLVED: To provide a micro-fluidic device for digital PCR with an adaptable flow rate of droplets, and a PCR method using the device. ! SOLUTION: A micro-fluidic device 100 for performing digital PCR is presented. The device comprises: a semiconductor substrate; a first micro-fluidic channel 104 embedded in the semiconductor substrate and having an inlet 102 and an outlet 103; a heating element 101 thermally coupled to the first micro-fluidic channel 104; and a droplet generator 107 connected to the inlet 102 of the first micro-fluidic channel 104 for generating droplets and pumping the generated droplets at a flow rate into the first micro-fluidic channel 104. The heating element 101 is a single heating element connected to a temperature control unit 111 configured to cycle the temperature of the complete first micro-fluidic channel 104 through at least two temperature values. The flow rate of the droplet generator 107 is adaptable. ! COPYRIGHT: (C)2015,JPO&INPIT