SYNTHESIS OF TANTALUM SOL-GEL AND PRODUCTION METHOD OF MICROEXTRACTION SYRINGE FOR THE PURPOSE OF ENRICHMENT OF PHOSPHOPEPTIDES

摘要

The present invention relates to a production method of sol-gel (100) comprising tantalum metal and a method (200) concerning use of the sol-gel produced for enrichment of phosphopeptides by means of a microextraction syringe. The syringe which is filled with the sol-gel comprising tantalum metal enables to make analysis of natural samples, that comprise low amount of phosphoprotein, with high precision and in a very short time and it shows compliance with mass spectrometric techniques. Thus, it can be used for application of fast and practical analytical methods for the analysis made in phosphoproteomics studies wherein mass spectrometric techniques are used.

主权项

1. A production method of sol-gel (100) wherein the steps comprising of:
adding one or more of tantalum (V) alkoxide, tantalum (V) methoxide, tantalum (V) ethoxide, tantalum (V) propoxide, tantalum (V) isopropoxide and tantalum (V) butoxide into the mixture (101); performing a mixing process during addition of material (102); adding an acid solution such as HNO3, HCl, H2SO4, CH3COOH, CF3COOH etc. during the mixing process (103); decomposing the sol-gel with tantalum content through a centrifuge process (104).