ADULT MESENCHYMAL STEM CELL CULTURE METHOD, KIT, COMPOSITION AND USES

摘要

The present invention describes a new adult mesenchymal stem cell culture method comprising the steps of: (a) incubating degenerated ex-vivo tissues in a culture medium; (b) isolating adult mesenchymal stem cells from the medium obtained in step (a); and (c) cultivating the adult mesenchymal stem cells isolated in step (b). The method further dispenses with the mechanical and enzymatic dissociation steps that are usually applied in the prior art. The present invention also provides an adult mesenchymal stem cell culture kit comprising: (a) a container made of an anti-adhesive material; (b) a suitable culture medium; and (c) a suitable cell culture container. The present invention additionally describes a composition comprising the adult mesenchymal stem cells obtained by the above-mentioned method, and a pharmaceutically acceptable carrier. The present invention further describes the use of the adult mesenchymal stem cells obtained by the above-mentioned method for producing a composition for the treatment of diskopathies involving degenerated intervertebral disks.