Microsieve Diagnostic Device In The Isolation and Analysis of Single Cells

摘要

A micro well plate is described for capturing and distributing single cells in individual wells is described, wherein at least one individual well is provided with a bottom plate having at least one pore to pass sample liquid, such that if one object or cell of interest is collected on the bottom plate of the well, the sample flow rate through that particular well is significantly reduced, minimizing the possibility that multiple cells or objects of interest entering the same well. The presented invention is particularly suited for obtaining single cells and/or microorganisms suspended in fluid samples for subsequent detailed interrogation.

主权项

1. A microwell plate for capturing an object of interest in a fluid sample comprising:
a. a microwell plate having individual microwells each with a bottom plate wherein at least one bottom plate has at least one precisely etched pore to pass sample liquid from a supply side to a discharge side; and b. a means to apply a fluid sample to the supply side wherein the fluid sample contains an object of interest with a slightly larger diameter than the pore such that when the sample fluid is applied to the microwell the object of interest will occlude the pore.