METHOD FOR IMAGING HIGH DENSITY BIOCHEMICAL ARRAYS WITH SUB-PIXEL ALIGNMENT

摘要

A method and associated system for imaging high density biochemical arrays comprises one or more imaging channels that share a common objective lens and a corresponding one or more time delay integration-type imaging cameras with optical alignment mechanisms that permit independent inter-channel and intra-channel adjustment of each of four degrees: X, Y, rotation and scale. The imaging channels are configured to independently examine different spectra of the image of the biochemical arrays.

主权项

1. A method for aligning an imaging system having a microscope objective and a multichannel imager, the method comprising:
(a) collecting light emitted from a sample on an x-y positioning stage; (b) obtaining an image of the sample along a rotational axis of symmetry of the microscope objective; (c) calculating a first angle between the multichannel imager and the sample around the rotational axis of symmetry; (d) calculating a second angle between the sample and the x-y positioning stage around the axis of rotational symmetry; (e) rotating the sample by the second angle to align the sample with the x-y positioning stage; and (f) rotating the multichannel imager by the sum of the first angle and the second angle to align the camera with the x-y positioning stage.