Device And Method For Detecting A Target Analyte

摘要

One aspect of the present disclosure relates to a calorimeter for detecting the presence of a target analyte in a fluid sample. The calorimeter can include a support structure, a hermetically-sealed, thermally decoupled central reaction zone associated with the support structure, at least one droplet transport region, and detection electronics. The at least one droplet transport region can be associated with the support structure and configured to merge a reagent droplet with a sample droplet including the fluid sample to form a reaction droplet in the central reaction zone. The detection electronics can be in electrical and/or thermal communication with the central reaction zone and associated with the support structure. The calorimeter can be configured to detect a heat of reaction produced by a reaction event between the target analyte and a capture reagent upon formation of the reaction droplet.

主权项

1. A method of detecting a target analyte in a fluid sample, using an analysis device including a supporting structure configured to receive an insert having a calorimeter and a surface which is capable of being functionalized with a specified bound analyte:
binding a sample to the specified bound analyte; applying a secondary capture agent including an enzyme to the functionalized surface; allowing the secondary capture agent to bind to the specified bound analyte; removing any unbound secondary capture agent from the functionalized surface; applying a substrate configured to react with at least one of the secondary capture agent and the sample generating a thermal change; and measuring a generated thermal change over a specified time period.