METHODS, SYSTEMS, AND COMPOSITIONS FOR CELL-DERIVED/VESICLE-BASED MICRORNA DELIVERY

摘要

Some embodiments comprise methods, systems, and compositions to produce and/or administer modified exosomes or other vesicles containing one or more selected microRNAs, including but not limited to, miR-146b. Some embodiments also comprise the therapeutic administration and use of such modified exosomes and/or producer cells to treat mammalian injuries and diseases, including in human beings.

主权项

1. A method of producing modified cell-derived membrane vesicles, comprising the steps of:
transfecting a cell population capable of producing cell-derived membrane vesicles with one or more plasmids encoding one or more microRNAs, harvesting cell-derived membrane vesicles from the cell population or media containing same after transfection, and confirming the presence of at least one of the microRNAs in one or more of the harvested cell-derived membrane vesicles.