METHOD OF DETECTING OXA-048 CARBAPENEMASE PRODUCING BACTERIA

摘要

A method of detecting and/or specifically identifying bacteria producing OXA-048-type carbapenemase in a biological sample, includes the steps of: (a) placing the biological sample likely to contain the bacteria in contact with a reaction medium including at least one chromogenic substrate to detect an enzymatic activity, and temocillin at a concentration equal to or greater than 150 mg/L, preferably between 200 and 500 mg/L, (b) incubating the sample in the medium to allow the bacteria to grow, and (c) detecting the strains corresponding to the OXA-48 carbapenemase producing bacteria. A culture medium as implemented in step (a) is also provided.

主权项

1. A method for specifically detecting and/or identifying OXA 48 carbapenemase-producing bacteria in a biological sample, comprising the steps of:
a) bringing the biological sample which may contain said bacteria into contact with a reaction medium comprising at least one chromogenic substrate which makes it possible to detect an enzymatic activity, and temocillin at a concentration greater than or equal to 150 mg/l, b) incubating the whole mixture so as to allow the bacteria to grow, and c) detecting the strains corresponding to the OXA 48 carbapenemase-producing bacteria.