NANOPORE SEQUENCING USING N-MERS

摘要

The invention relates to devices and methods for nanopore sequencing. The invention provides for using the signals from n-mers to provide sequence information, for example where the system has less than single base resolution. The invention includes arrays of nanopores having incorporated electronic circuits, for example, in CMOS. In some cases, the arrays of nanopores comprise resistive openings for isolating the electronic signals for improved sequencing. Methods for controlling translocation of through the nanopore are disclosed.

主权项

1. A method for sequencing a nucleic acid molecule with a nanopore system comprising:
providing a substrate comprising a nanopore in contact with a solution, the solution comprising a template nucleic acid above the nanopore, wherein at least a portion of the sequence of the template nucleic acid is unknown; providing a voltage across the nanopore; measuring the current through the nanopore over time as the template nucleic acid is translated through the nanopore; determining a sequence of the nucleic acid template by comparing the measured current through the nanopore over time with known current over time values for n-mers.