| 发明名称 |
ENGINEERED CLEAVAGE HALF-DOMAINS |
| 摘要 |
Disclosed herein are engineered cleavage half-domains; fusion polypeptides comprising these engineered cleavage half-domains; polynucleotides encoding the engineered cleavage half-domains and fusion proteins; and cells comprising said polynucleotides and/or fusion proteins. Also described are methods of using these polypeptides and polynucleotides, for example for targeted cleavage of a genomic sequence. |
| 申请公布号 |
US2015159176(A1) |
申请公布日期 |
2015.06.11 |
| 申请号 |
US201514627812 |
申请日期 |
2015.02.20 |
| 申请人 |
SANGAMO BIOSCIENCES, INC. |
发明人 |
Doyon Yannick;Miller Jeffrey C. |
| 分类号 |
C12N15/90;C07K14/47;C12N9/22 |
主分类号 |
C12N15/90 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A fusion protein comprising a polypeptide comprising an engineered FokI cleavage half-domain, wherein the engineered cleavage half-domain comprises a mutation selected from the group consisting of: mutations in amino acid residues 486, 499 and 496; mutations in amino acid residues 487 and 496; mutations in amino acid residues 487, 499, and 496; mutations in amino acid residues 483 and 537; mutations in amino acid residues 490 and 537; mutations in amino acid residues 490, 537 and 538; mutations in amino acid residues 483 and 538; mutations in amino acid residues 487, 499 and 496; and mutations in amino acid residues 483, 538 and 537, wherein the amino acid residues are numbered relative to full length wild-type FokI. |
| 地址 |
RICHMOND CA US |
