METHOD OF SUBSPECIES DIFFERENTIATION OF PLAGUE AGENT STRAINS USING POLYMERASE CHAIN REACTION METHOD

摘要

FIELD: biotechnologies.SUBSTANCE: method provides DNA extraction, conducting of PCR with the use of synthesized primers for amplification of fragments of intergenic segments wzyE-dapF and YPDF_0064-YPDSF_0065 of the studied strain. Differentiation is conducted by comparison of sizes of produced amplicons of the studied strain with the sizes of similar fragments, typical for strains of the basic (151 p. n. and 374 p. n.), Caucasian (185 p. n. and 434 p. n.), Altai and Hissar (202 p. n. and 374 p. n.), and also Ulegey (185 p. n. and 374 p. n.) subspecies.EFFECT: invention allows to conduct effectively subspecies differentiation of strains and considerably to reduce time of the analysis execution.4 ex