包含微生物蛋白酶变体的组合物和方法

摘要

本发明提供枯草杆菌蛋白酶变体和包含至少一种此处陈列的枯草杆菌蛋白酶变体的组合物，以及使用这些变体和组合物的方法。在一些实施方案中，本发明提供适于衣物清洁用途的枯草杆菌蛋白酶变体。

主权项

分离的枯草杆菌蛋白酶变体，包含以下取代组中的至少一组：G97A‑G128A‑Y217Q、G97A‑L126A‑G128A、G97A‑L126A‑G128A‑Y217Q、G97A‑L126A‑Y217Q、G97A‑M124V‑G128A、G97A‑M124V‑G128A‑Y217Q、G97A‑M124V‑L126A、G97A‑M124V‑L126A‑G128A、G97A‑M124V‑L126A‑Y217Q、G97A‑M124V‑Y217Q、G97A‑N123G‑G128A、G97A‑N123G‑G128A‑Y217Q、G97A‑N123G‑L126A、G97A‑N123G‑L126A‑G128A、G97A‑N123G‑L126A‑Y217Q、G97A‑N123G‑M124V、G97A‑N123G‑M124V‑G128A、G97A‑N123G‑M124V‑L126A、G97A‑N123G‑M124V‑Y217Q、G97A‑N123G‑Y217Q、L126A‑G128A‑Y217Q、L96T‑G128A‑Y217Q、L96T‑G97A‑G128A、L96T‑G97A‑G128A‑Y217Q、L96T‑G97A‑L126A、L96T‑G97A‑L126A‑G128A、L96T‑G97A‑L126A‑Y217Q、L96T‑G97A‑M124V、L96T‑G97A‑M124V‑G128A、L96T‑G97A‑M124V‑L126A、L96T‑G97A‑M124V‑Y217Q、L96T‑G97A‑N123G、L96T‑G97A‑N123G‑G128A、L96T‑G97A‑N123G‑L126A、L96T‑G97A‑N123G‑M124V、L96T‑G97A‑N123G‑Y217Q、L96T‑G97A‑Y217Q、L96T‑L126A‑G128A、L96T‑L126A‑G128A‑Y217Q、L96T‑L126A‑Y217Q、L96T‑M124V‑G128A、L96T‑M124V‑G128A‑Y217Q、L96T‑M124V‑L126A、L96T‑M124V‑L126A‑G128A、L96T‑M124V‑L126A‑Y217Q、L96T‑M124V‑Y217Q、L96T‑N123G‑G128A、L96T‑N123G‑G128A‑Y217Q、L96T‑N123G‑L126A、L96T‑N123G‑L126A‑G128A、L96T‑N123G‑L126A‑Y217Q、L96T‑N123G‑M124V、L96T‑N123G‑M124V‑G128A、L96T‑N123G‑M124V‑L126A、L96T‑N123G‑M124V‑Y217Q、L96T‑N123G‑Y217Q、M124V‑G128A‑Y217Q、M124V‑L126A‑G128A、M124V‑L126A‑G128A‑Y217Q、M124V‑L126A‑Y217Q、N123G‑G128A‑Y217Q、N123G‑L126A‑G128A、N123G‑L126A‑G128A‑Y217Q、N123G‑L126A‑Y217Q、N123G‑M124V‑G128A、N123G‑M124V‑G128A‑Y217Q、N123G‑M124V‑L126A、N123G‑M124V‑L126A‑G128A、N123G‑M124V‑L126A‑Y217Q、N123G‑M124V‑Y217Q、N62Q‑G128A‑Y217Q、N62Q‑G97A‑G128A、N62Q‑G97A‑G128A‑Y217Q、N62Q‑G97A‑L126A、N62Q‑G97A‑L126A‑G128A、N62Q‑G97A‑L126A‑Y217Q、N62Q‑G97A‑M124V、N62Q‑G97A‑M124V‑G128A、N62Q‑G97A‑M124V‑L126A、N62Q‑G97A‑M124V‑Y217Q、N62Q‑G97A‑N123G、N62Q‑G97A‑N123G‑G128A、N62Q‑G97A‑N123G‑L126A、N62Q‑G97A‑N123G‑M124V、N62Q‑G97A‑N123G‑Y217Q、N62Q‑G97A‑Y217Q、N62Q‑L126A‑G128A、N62Q‑L126A‑G128A‑Y217Q、N62Q‑L126A‑Y217Q、N62Q‑L96T‑G128A、N62Q‑L96T‑G128A‑Y217Q、N62Q‑L96T‑G97A、N62Q‑L96T‑G97A‑G128A、N62Q‑L96T‑G97A‑L126A、N62Q‑L96T‑G97A‑M124V、N62Q‑L96T‑G97A‑N123G、N62Q‑L96T‑G97A‑Y217Q、N62Q‑L96T‑L126A、N62Q‑L96T‑L126A‑G128A、N62Q‑L96T‑L126A‑Y217Q、N62Q‑L96T‑M124V、N62Q‑L96T‑M124V‑G128A、N62Q‑L96T‑M124V‑L126A、N62Q‑L96T‑M124V‑Y217Q、N62Q‑L96T‑N123G、N62Q‑L96T‑N123G‑G128A、N62Q‑L96T‑N123G‑L126A、N62Q‑L96T‑N123G‑M124V、N62Q‑L96T‑N123G‑Y217Q、N62Q‑L96T‑Y217Q、N62Q‑M124V‑G128A、N62Q‑M124V‑G128A‑Y217Q、N62Q‑M124V‑L126A、N62Q‑M124V‑L126A‑G128A、N62Q‑M124V‑L126A‑Y217Q、N62Q‑M124V‑Y217Q、N62Q‑N123G‑G128A、N62Q‑N123G‑G128A‑Y217Q、N62Q‑N123G‑L126A、N62Q‑N123G‑L126A‑G128A、N62Q‑N123G‑L126A‑Y217Q、N62Q‑N123G‑M124V、N62Q‑N123G‑M124V‑G128A、N62Q‑N123G‑M124V‑L126A、N62Q‑N123G‑M124V‑Y217Q、和N62Q‑N123G‑Y217Q，其中所述取代位于SEQ ID NO:1中陈列的BPN’枯草杆菌蛋白酶位置的等同位置。