发明名称 |
DETECTION OF BLOOD PLASMA SCHIZANDRIN B OF DISSIPATING BLOOD STASIS BOTANICAL |
摘要 |
<p>A detection method of blood plasma schizadrin B of dissipating blood stasis botanical is disclosed. The method includes: (1) extracting schizadrin B from plasma of mammalian administered dissipating blood stasis botanical by ethyl acetate with the volume ratio 1:4, whirling 3-5mins, centrifugating at 9600rpm for 10mins, drying and enriching the upper layer at 25-30°C, and redissolving with mobile phase; (2) UPLC/MS measuring: UPLC condition: chromatographic column: Acquity UPLC BEH C 18 , 2.1'100mm, mobile phase A: water-acetonitrile-formic acid 95:5:0.1 v/v/v, mobile phase B: acetonitrile-formic acid 100:0.1 v/v; MS condition: electric spraying ion source(ESI), detecting with positive ion mode, scanning at the range of m/z 150-1200. The method can be used for pharmacokinetics study of schizadrin B in dissipating blood stasis botanical.</p> |
申请公布号 |
EP2146206(B1) |
申请公布日期 |
2015.06.10 |
申请号 |
EP20080748424 |
申请日期 |
2008.04.28 |
申请人 |
SHANGHAI SUNDISE CHINESE MEDICINE TECHNOLOGY DEVELOPMENT CO., LTD;MA, YUEMING;SHI, RONG;ZHANG, YONGYU |
发明人 |
MA, YUEMING;SHI, RONG;ZHANG, YONGYU |
分类号 |
G01N33/94;G01N30/36;G01N30/72;G01N33/15;G01N33/49 |
主分类号 |
G01N33/94 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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