| 摘要 |
FIELD: medicine.SUBSTANCE: invention is intended for prevention of post-infectious irritable bowel syndrome (PI-IBS). Peroral intake of autoprobiotic, representing individual lactic product, prepared on the basis of Enterococcus faecium strain, and containing 108 CFU in 1 ml of product. Inoculation of bacteria from patient's feces on selective nutritional medium is performed, with further culture growing in selective nutritional medium, identification and selection of colonies, typical for Enterococcus faecium, obtaining pure culture of Enterococcus faecium and specification of species and content of pathogenecity factors by method of polymerase chain reaction, selection of apatogenic clones of Enterococcus faecium, corresponding to standards of genetic safety and physiological functionality, which are used to prepare lactic starter culture. Autoprobiotic is introduced in dose not less than 100 ml/day, with course constituting not fewer than 10 days. Selected apathogenic clones of Enterococcus faecium are deposited in cryostorage at temperature not higher than -75°C with storage term not longer than 1 year. Enterococcus-agar is used as selective nutritional medium.EFFECT: method makes it possible to increase efficiency of prophylaxis of PI-IBS patients due to reduction of its development frequency.3 cl, 1 tbl, 1 ex |
