Image classification of germination potential of somatic embryos

摘要

The present invention is directed towards methods for the classification of plant embryos by the application of one or more classification algorithms to analyze digitized images and absorption, transmittance, or reflectance spectra. The methods are generally applicable and emphasize the importance of acquiring and using as much image and absorption, transmittance, or reflectance spectral information as possible, based on objective criteria. The present invention allows automated selection of embryos most suitable for further culture and rejection of those seen as less suitable.

主权项

1. A method for classifying plant embryos according to their germination potential, comprising:
(a) developing a classification model by
(i) acquiring raw digital image data of reference samples of whole plant embryos or of embryo organs of known germination potential;(ii) performing a data analysis by applying one or more classification algorithms to the acquired raw digital image data, wherein at least one of the one or more classification algorithms uses non-perimeter information, the non-perimeter information being more than an embryo perimeter from the acquired raw digital image data, the data analysis resulting in development of a classification model for classifying plant embryos by their germination potential; (b) acquiring raw digital image data of a plant embryo or a plant embryo organ of unknown germination potential; and (c) applying the developed classification model to the raw digital image data of step (b) in order to classify the plant embryo of unknown germination potential according to its presumed germination potential.