Method for processing and embedding tissue

摘要

A method for treating a tissue sample including placing at least one tissue sample on an cassette which has: a retaining member, a base and at least one biasing element; placing the at least one tissue sample in the tissue cassette; attaching the base and the retaining member to retain the tissue sample; processing the tissue sample in the tissue cassette with one or more solvents; and embedding the tissue sample in a paraffin to form an portion of paraffin in which the tissue sample is embedded in the tissue cassette, wherein the embedding comprises adding molten paraffin to the interior area of the tissue cassette and allowing the paraffin to become solid.

主权项

1. A method for treating a tissue sample, comprising
providing a tissue retaining cassette, comprising:
a retaining member having an first tissue engaging surface and at least one biasing element, the first tissue engaging surface being moveably attached to the retaining member by said biasing element, and a base comprising a second tissue engaging surface and configured to engage the retaining member to form an interior area with the first and second tissue engaging surfaces facing each other; placing the at least one tissue sample on one of the first tissue engaging surface and the second tissue engaging surface; attaching the base and the retaining member to cause the biasing element to urge first tissue engaging surface towards the second tissue engaging surface, such that the tissue sample is held between the tissue engaging surfaces; processing the tissue sample in the tissue cassette with one or more solvents; and embedding the tissue sample in paraffin while the tissue is in the tissue cassette.