Substrate independent copolymers for biofunctionalization

摘要

The present invention provides crosslinked epoxy-functional copolymer films and microarrays built from the crosslinked epoxy-functional copolymer films. Microarrays incorporating the copolymers include a substrate on which a film of the crosslinked epoxy-functional copolymer is disposed and target molecules bound to the copolymer film. The crosslinked polymer films are well-suited for use as scaffolds for target molecules in microarrays because they provide a high density of binding sites for the target molecules, are mechanically stable, and may be coated onto a wide range of substrates.

主权项

1. A microarray comprising:
(a) a substrate; and (b) a copolymer film disposed on the substrate, the copolymer film comprising a first set of polymerized monomers bearing an epoxy group reacted with a target molecule and a second set of polymerized photocrosslinked 2-(acryloyloxy)ethyl 2-methylacrylate monomers; wherein the copolymer film has a thickness of no more than about 100 nm and further wherein the thickness of the copolymer film is reduced by no more than 5%, as measured by ellipsometry after immersion in deionized water at 50° C. for two weeks.