| 主权项 |
1. An immunogenic composition comprising an isolated and inactivated EV71-E59 virus full-particle and an isolated and inactivated EV71-E59 virus sub-particle, the inactivated full particle and the inactivated sub-particle being produced by incubating an EV71-E59 virus full-particle and an EV71-E59 virus sub-particle in a 37% formaldehyde solution, wherein the inactivated full-particle and the inactivated sub-particle each contain viral antigens that are cross-linked by the formaldehyde solution, and wherein, prior to being inactivated, (a) the full particle contains an EV71-E59 VP1 protein having a molecular weight of 36 kDa, an EV71-E59 VP2 protein having a molecular weight of 28 kDa, an EV71-E59 VP3 protein having a molecular weight of 27 kDa, and an EV71-E59 VP4 protein having a molecular weight of 8 kDa; (b) the sub-particle contains an EV71-E59 VP0 protein having a molecular weight of 38 kDa, the EV71-E59 VP1 protein having a molecular weight of 36 kDa, and the EV71-E59 VP3 protein having a molecular weight of 27 kDa; and (c) in a 10-50% continuous sucrose gradient, the full particle is detected in fractions having 35 to 38% sucrose and the sub-particle is detected in fractions having 25 to 28% sucrose, the molecular weights of the proteins being observed molecular weights on a 10% SDS-polyacrylamide gel. |
