MUTERENDE DELTA8-DESATURASEGENER KONSTRUERET VED MÅLRETTET MUTAGENESE OG ANVENDELSE HERAF I FREMSTILLINGEN AF FLERUMÆTTEDE FEDTSYRER

摘要

The present invention relates to mutantΔ8 desaturase genes, which have the ability to convert eicosadienoic acid [20:2ω-6, EDA] to dihomo-γ-linolenic acid [20:3, DGLA] and/or eicosatrienoic acid [20:3ω-3, ETrA] to eicosatetraenoic acid [20:3ω-3, ETA]. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encodingΔ8 desaturase along with methods of making long-chain polyunsaturated fatty acids (PUFAs) using these mutantΔ8 desaturases in plants and oleaginous yeast are disclosed.