| 主权项 |
1. A method of co-producing butadiene and 1-propanol and/or 1,2-propanediol from a fermentable carbon source, the method comprising:
providing a fermentable carbon source; contacting the fermentable carbon source with a microorganism comprising one or more polynucleotides coding for enzymes in a pathway that catalyzes a conversion of the fermentable carbon source to one or more intermediates in a pathway for the co-production of butadiene and 1-propanol and/or 1,2-propanediol, and one or more polynucleotides coding for enzymes in a pathway that catalyze a conversion of the one or more intermediates to butadiene and 1-propanol and/or 1,2-propanediol in a fermentation media; and expressing the one or more polynucleotides coding for the enzymes in the pathway that catalyzes a conversion of the fermentable carbon source to one or more intermediates in a pathway for the co-production of butadiene and 1-propanol and/or 1,2-propanediol and one or more polynucleotides coding for enzymes in a pathway that catalyze a conversion of the one or more intermediates to butadiene and 1-propanol and/or 1,2-propanediol in the microorganism to produce butadiene and 1-propanol and/or 1,2-propanediol, wherein the one or more intermediates in the pathway for the production of butadiene are selected from the group consisting of: crotonyl alcohol, 5-hydroxy-3-ketovaleryl-CoA, 3-ketopent-4-enoyl-CoA and 3,5-ketovaleryl-CoA, wherein the one or more intermediates in the pathway for the production of 1-propanol and/or 1,2-propanediol are selected from the group consisting of: methylglyoxal and lactate, and wherein the co-production method is anaerobic. |
