FULL AND PARTIAL PROTEIN SECRETION AND CELL SURFACE DISPLAY USING TYPE III SECRETION SYSTEM

摘要

The present invention provides a cell which fully or partially secretes a protein of interest via a type III secretion system (T3SS). The cells comprising a type III secretion system and a protein of interest with having an N-terminal secretion tag. Partial secretion is achieved by using an anchoring sequence which plugs the entry point of the T3SS to prevent the complete secretion of the protein of interest.