| 摘要 |
PROBLEM TO BE SOLVED: To provide a PCR clamping method, specifically in the PCR clamping method for bacterial flora analysis in a plant sample, a method with higher general-purpose properties.SOLUTION: When one or more kinds of PNA probes which inhibit a PCR reaction of DNA derived from plants, and do not inhibit a PCR reaction derived from microorganisms use the DNA derived from plants having a sequence complementary to the sequence of a sense strand or an antisense strand contained in a region between a position of 30 bases or more downstream from the 3'-terminal side of a primer in a region where a forward primer anneals and a position of 30 bases or more downstream from the 3'-terminal side of a primer in a region where a reverse primer anneals, the above subject is solved. |
