SIMIAN ADENOVIRUSES SAdV-43, -45, -46, -47, -48, -49, AND -50, AND USES THEREOF

摘要

A recombinant vector comprises simian adenovirus 43, 45, 46, 47, 48, 49 or 50 sequences and a heterologous gene under the control of regulatory sequences. A cell line which expresses simian adenovirus 43, 45, 46, 47, 48, 49 or 50 gene(s) is also disclosed. Methods of using the vectors and cell lines are provided.

主权项

1. A recombinant adenovirus having an adenovirus capsid comprising a capsid protein selected from the group consisting of:
(a) a hexon protein of SAdV-43, amino acids 1 to 955 of SEQ ID NO: 11; a hexon protein of SAdV-45, amino acids 1 to 953 of SEQ ID NO: 48; a hexon protein of SAdV-47, amino acids 1 to 958 of SEQ ID NO: 109; a hexon protein of SAdV-48, amino acids 1 to 934 of SEQ ID NO: 139; a hexon protein of SAdV-49, amino acids 1 to 916 of SEQ ID NO: 167; a hexon protein of SAdV-50, amino acids 1 to 920 of SEQ ID NO: 193; (b) a penton protein of SAdV-46, amino acids 1 to 563 of SEQ ID NO: 74; a penton protein of SAdV-43, amino acids 1 to 651 of SEQ ID NO: 6; a penton protein of SAdV-45, amino acids 1 to 664 of SEQ ID NO: 43; a penton protein of SAdV-47, amino acids 1 to 580 of SEQ ID NO: 104; a penton protein of SAdV-48, amino acids 1 to 504 of SEQ ID NO: 134; a penton protein of SAdV-49, amino acids 1 to 511 of SEQ ID NO: 162; a penton protein of SAdV-50, amino acids 1 to 511 of SEQ ID NO: 188; and (c) a fiber protein of SAdV-46, amino acids 1 to 353 of SEQ ID NO: 89; a fiber protein of SAdV-43, amino acids 1 to 599 of SEQ ID NO: 22; a fiber protein of SAdV-45, amino acids 1 to 601 of SEQ ID NO: 59; a fiber protein of SAdV-47, amino acids 1 to 322 of SEQ ID NO: 119; a fiber protein of SAdV-48, amino acids 1 to 570 of SEQ ID NO: 147; a fiber protein of SAdV-49, amino acids 1 to 521 of SEQ ID NO: 174; a fiber protein of SAdV-49, amino acids 1 to 418 of SEQ ID NO: 175; a fiber protein of SAdV-50, amino acids 1 to 521 of SEQ ID NO: 200; a fiber protein of SAdV-50, amino acids 1 to 418 of SEQ ID NO: 201; said capsid encapsidating a heterologous molecule carrying a gene operably linked to expression control sequences which direct transcription, translation, and/or expression thereof in a host cell.