发明名称 |
METHODS FOR CULTURE AND PRODUCTION OF SINGLE CELL POPULATIONS OF HUMAN EMBRYONIC STEM CELLS (HESCS) |
摘要 |
We used ACCUTASE®, a commercially available cell detachment solution, for single cell propagation of pluripotent hESCs. Unlike trypsin dissociation, ACCUTASE® treatment does not significantly affect the plating efficiency of hESC dissociation into single cells. Cultures dissociated with ACCUTASE® to single cells at each passage maintain a higher proportion of pluripotent cells as compared to collagenase-passaged hESCs. ACCUTASE®-treated hESCs can be grown to a high density as monolayers, and yet retain their pluripotency. |
申请公布号 |
US2015152380(A1) |
申请公布日期 |
2015.06.04 |
申请号 |
US201414554045 |
申请日期 |
2014.11.26 |
申请人 |
BURNHAM INSTITUTE FOR MEDICAL RESEARCH |
发明人 |
Terskikh Alexey;Bajpai Ruchi |
分类号 |
C12N5/0735;G01N33/569;G01N33/50 |
主分类号 |
C12N5/0735 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
La Jolla CA US |