| 发明名称 |
CELLS DEFICIENT IN CMP-N-ACETYLNEURAMINIC ACID HYDROXYLASE AND/OR GLYCOPROTEIN ALPHA-1,3-GALACTOSYLTRANSFERASE |
| 摘要 |
The present invention provides non-human mammalian cell lines that are deficient in CMP-Neu5Ac hydroxylase (Cmah) and/or glycoprotein alpha-1,3-galactosyltransferase (Ggta1). Also provided are methods for using the cells disclosed herein for producing recombinant proteins with human-like patterns of glycosylation. |
| 申请公布号 |
US2015152463(A1) |
申请公布日期 |
2015.06.04 |
| 申请号 |
US201514609603 |
申请日期 |
2015.01.30 |
| 申请人 |
Lin Nan;George Henry J.;Mascarenhas Joaquina;Collingwood Trevor N.;Kayser Kevin J.;Achtien Katherine |
发明人 |
Lin Nan;George Henry J.;Mascarenhas Joaquina;Collingwood Trevor N.;Kayser Kevin J.;Achtien Katherine |
| 分类号 |
C12P21/00 |
主分类号 |
C12P21/00 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method for producing a recombinant protein with a human-like glycosylation pattern, the method comprising expressing the recombinant protein in a non-human mammalian cell line deficient in cytidine monophosphate-N-acetylneuraminic acid hydroxylase (Cmah) and/or glycoprotein alpha-1,3-galactosyltransferase-1 (Ggta1). |
| 地址 |
St. Louis MO US |
