发明名称 |
COMPOSITIONS AND METHODS FOR PROTEIN PRODUCTION |
摘要 |
The present invention provides a method of selecting a mRNA for production of a polypeptide of interest comprising: a) producing an array of individual mRNA sequences comprising different nucleotide sequences encoding the polypeptide of interest; b) determining one or more or two or more of the following parameters for each individual mRNA sequence of (a): (i) minimum free energy (MFE) RNA secondary structure; (ii) ensemble free energy (EFE); (iii) frequency of the minimum free energy (FMFE) RNA secondary structure in a thermodynamic ensemble; and (iv) ensemble diversity (ED); c) ranking the individual mRNA sequences of the array according the parameters determined in step (b); and d) selecting a mRNA sequence from the ranked array of step (c), wherein the selected mRNA produces the polypeptide of interest. The present invention further provides a method of selecting a mRNA for enhanced and reduced production of a polypeptide of interest. |
申请公布号 |
US2015152408(A1) |
申请公布日期 |
2015.06.04 |
申请号 |
US201514593707 |
申请日期 |
2015.01.09 |
申请人 |
Syngenta Participations AG |
发明人 |
Basu Shib Sankar;Dang Kristen Kamerath;Azhakanandam Kasimalai;McNeill Thomas Z.;Guo Sheng |
分类号 |
C12N15/10 |
主分类号 |
C12N15/10 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
Basel CH |