NUCLEIC ACID MOLECULES AND COLLECTIONS THEREOF, THEIR APPLICATION AND MODIFICATION

摘要

The invention provides a method for characterising a sample comprising nucleic acid derived from a cell. The method comprises determining whether a sample comprises at least a minimal sequence of at least one new microRNA (miRNA) according to the invention or a mammalian ortholog thereof and characterizing the sample on the basis of the presence or absence of the miRNA. The invention further provides nucleic acid molecules and collections thereof and their use in therapeutic and diagnostic applications. The invention furthermore provides a method for identifying a miRNA molecule or a precursor molecule thereof.

主权项

1. An isolated nucleic acid molecule, wherein said nucleic acid molecule is a miRNA, a pre-miRNA or a DNA molecule encoding said pre-miRNA, which has a length of 18-26 nucleotides in the case of miRNA, wherein:
(a) the sequence of the nucleic acid molecule comprises SEQ ID NO: 11123 or its corresponding ribonucleotide sequence, or (b) the sequence of the nucleic acid molecule is at least 80% identical to the miRNA sequence of (a),wherein said sequence comprises at least one modification selected from the group consisting of:a nucleotide analogue, a peptide nucleic acid, a locked nucleic acid, a backbone modified ribonucleotide or deoxyribonucleotide and a sugar modified ribonucleotide or deoxyribonucleotide.