Method of Normalizing Biological Samples

摘要

The present disclosure relates to normalization of biological samples, particularly samples comprising nucleic acids to be sequenced. The normalization protocols described herein may be utilized across multiple samples to cap total stoichiometric input and minimize variations in transcript abundance on a per-sample basis in a multiplexed fashion to dramatically increase the accuracy, capacity and efficiency of nucleic acid sequencing.

主权项

1. A method of normalizing biological samples, the method comprising:
obtaining multiple samples, each sample comprising a plurality of targets; attaching capture tags to substantially all of the targets in a given sample, wherein each capture tag comprises an identifying feature and capture moiety-binding domain, both of which are unique to the sample to which it is added; pooling the samples; after or optionally before pooling, adding an amount of a capture moiety for each sample, wherein each capture moiety is specific for the capture moiety-binding domain of the capture tag utilized in a given sample, and wherein the total amount of each sample-specific capture moiety
a. is equivalent across all samples, andb. is less than at least the most abundanttarget in any one of the samples; and capturing the targets.