| 摘要 |
Disclosed is an industrial preparation of natural killer cells (NKs) and an injection using human allogeneic karyocytes comprising: using umbilical cord blood and peripheral blood from legitimate sources as raw materials, obtaining stem cells by a method for extracting and separating karyocytes, or using Ficoll or percoll density gradient centrifugation to isolate and screen out karyocytes; diluting the above-mentioned karyocytes with cell culture medium, adding interferon, interleukin, CD3 antibody, and human albumin, loading them together into a bioreactor for perfusion culture, and then performing multiplication culture; the passage number of natural killer cells from multiplication culture is no less than 8, and the culture time is no less than 4 weeks; the markers of the natural killer cells obtained after the multiplication culture are CD3 - /CD56 + , CD16 + , CD57 + , and CD8 + , wherein CD16 + /CD56 + ‰¥15%, CD3 - /CD56 + ‰¥ 50%, and CD8 + /CD57 + ‰¥ 8%; then preparing an injection with a certain concentration using the cell suspension obtained by above-mentioned method. |
