Isolated nucleotide molecule and method of sensing and killing of pathogenic microorganism

摘要

The present invention relates to an isolated nucleic acid molecule comprising a first nucleotide sequence encoding a protein that detects the presence, amount or both of a pathogenic microorganism by forming a complex with a protein produced by said pathogenic microorganism; a second nucleotide sequence encoding an antimicrobial peptide, wherein the antimicrobial peptide is effective against the pathogenic microorganism detected by the protein encoded by the first nucleotide sequence, wherein the second nucleotide sequence is under control of a promoter that is induced by the complex of the protein encoded by the first nucleotide sequence and the protein produced by said pathogenic microorganism. A recombinant microorganism comprising the isolated nucleic acid molecule and a method of sensing and killing pathogenic microorganisms is also described.

主权项

1. An isolated nucleic acid molecule comprising:
a first nucleotide sequence encoding a protein that is able to form a complex with an acyl homoserine lactone (AHL) produced by a pathogenic microorganism; and a second nucleotide sequence encoding an antimicrobial peptide, wherein the antimicrobial peptide is effective against the pathogenic microorganism, wherein the second nucleotide sequence is under control of a promoter that is induced by the complex of the protein encoded by the first nucleotide sequence and the acyl homoserine lactone (AHL) produced by said pathogenic microorganism, and wherein the protein encoded by the second nucleotide sequence is a bacterocin.