Cross-reactive determinants and methods for their identification

摘要

Compositions and methods for determining immunologically cross-reactive molecules comprising a cross-reactive antigenic determinant are provided, in particular for determining proteins comprising cross-reactive antigenic determinants, in particular for determining proteins that are cross-reactive based on serological screens using sequential immunological challenges to an animal, including determining cross-reactive H. parasuis proteins. Also provided are compositions, vaccines, and kits using the molecules for diagnostics and methods for preventing or treating a disease, disorder, condition, or symptoms thereof associated with infectious agents, in particular infectious microorganisms, in particular for preventing or treating a disease, disorder, condition, or symptom thereof associated with H. parasuis infection.

主权项

1. An isolated polypeptide comprising an amino acid sequence selected from the group consisting of: TVLAEKQEII (SEC) ID NO:2); APAKGSTIEAGIAYPIST (SEQ ID NO:3); and SPSDKTFKISAIPDYNAAEMT (SEQ ID NO:5), wherein the isolated polypeptide further comprises a cross-reactive antigenic determinant present in a protein expressed by at least two serotypes of H. parasuis, and wherein the isolated polypeptide is linked to a carrier substance selected from the group consisting of N-succcidimidyl-3-(2-pyridyldithio)proprionate (SPDP), a reactive ester of 6-maleimidocaproic acid, a reactive ester of 2 bromnoacetic acid, a reactive ester of 2-iodoacetic acid, a reactive ester of 4-(N-maleimido-methyl)cyclohexane-1-carboxylic acid, a cysteine residue, and an N-terminal His Tag; or wherein the isolated polypeptide is an acid addition salt formed with the free amino group of the peptide or a base addition salt formed with the free carboxyl of the peptide.