Inkjet gene printing

摘要

Provided herein are methods and apparatuses for transfecting a cell with a compound of interest by stressing the cell, e.g. with shear stress. The compound of interest may be nucleic acids, proteins, molecules, nanoparticles, drugs, etc., or any combination thereof. Methods of printing cells with an inkjet printing device are also provided, wherein at least a portion of viable cells (preferably at least 1%) are transfected with a compound of interest. Preferably, at least 25% of the cells are viable after printing. In addition, methods of forming an array of viable cells are provided wherein at least a portion of the viable printed cells (preferably at least 1%) are transfected with at least one compound of interest.

主权项

1. A method for transfecting cells with a nucleic acid, wherein at least a portion of said cells are transfected with said nucleic acid, said method comprising the steps of:
providing a composition comprising said cells in the presence of said nucleic acid in a liquid carrier; and forcing said composition through an orifice so that said cells undergo a shear stress of from 0.5 to 500 ms−1 in the presence of said nucleic acid, wherein said orifice has a diameter of between one-eighth and twelve times the average diameter of said cells, and wherein said cells are stressed for a period of from 0.1 to 10 microseconds; thereby transfecting at least a portion of said cells with said nucleic acid.