DNA extraction using a photo-polymerized monolith in a capillary

摘要

The present invention relates to the fabrication of a grafted, UV photo-polymerized silica-based monolithic column and the use of such column for the extraction of DNA. In one embodiment, a method is provided for fabricating a silica-based monolithic column, wherein a vessel is filled with a polymerization mixture that is formed into monolithic solid phase for DNA extraction through in situ photo-polymerization.

主权项

1. A method for preparing a monolithic column comprising the steps of:
(i) providing a vessel; (ii) conditioning the interior surface of said vessel by contacting it with a silica-based flushing solution; (iii) preparing a silica-based monomer solution through hydrolysis of a silica monomer; (iv) forming a polymerizable mixture by admixing said silica-based monomer solution, an initiator material, and a porogenic solvent; (v) introducing said polymerizable mixture into the vessel; (vi) initiating the in situ polymerization of said polymerizable mixture by exposing selected portions of the mixture to UV light, thereby forming the UV photo-polymerized silica-based monolithic column within portions of the vessel; (vii) flushing a silica-based monomer reagent through said UV photo-polymerized silica-based monolithic column, thereby forming the grafted porous UV photo-polymerized silica-based monolithic column within portions of the vessel.