Device for capture and lysis of microorganisms from liquids

摘要

Devices and methods for detecting microbial contaminants, such as bacteria and fungi, in fluids such as drinking water, pharmaceutical solutions and tissue culture media are provided. More particularly, provided are filtration devices for capture and processing of microorganisms from fluids, and improved methods for recovery, lysis and detection of microorganisms based on a combination of physical disruption with small beads and lysis solutions.

主权项

1. A device for capture and lysis of microorganisms from a liquid comprising:
a) a filter housing consisting of a hollow body defining a fluid path, having an upper input end adapted for receiving a liquid, a lower outflow end, and a filter cavity therebetween; b) a filter, having an input face and an output face, secured within the filter cavity in an orientation perpendicular to the fluid path wherein the filter is configured such that fluid can enter the input end of the housing and then pass through the filter before exiting the output end, wherein the filter divides the filter cavity into an upper input cavity between the input end of the housing and the input face of the filter, and a lower output cavity between the output face of the filter and the output end of the housing; c) at least one bead disposed within the input cavity, wherein the bead can move freely within the input cavity when the device is agitated and the bead is about 10 μm to about 100 μm in diameter; d) a first sealing means for removably sealing the input end, wherein the first sealing means is selected from a cap and a plug; and e) a second sealing means for removably sealing the output end, wherein the second sealing means is selected from a cap and a plug.