Method of reconstituting cellular spectra useful for detecting cellular disorders

摘要

Embodiments of the present disclosure provides improved methods for determining the presence of abnormalities in exfoliated cells. In one embodiment, the present disclosure provides methods for reconstructing cellular spectrum of a cell sample by creating a spectral map of the cellular sample, generating a binary mask of the spectral map, removing edge artifacts from each cell, and co-adding spectral data of each pixel corresponding to the cell to reconstruct the spectrum of each cell.

主权项

1. A method of generating a spectrum of a cell, the method comprising:
receiving a plurality of spectral pixels, each spectral pixel corresponding to a portion of a sample and being associated with a plurality of intensity measurements, each intensity measurement representing an intensity of light associated with a wavenumber, wherein one of the intensity measurements associated with each spectral pixel corresponds to one characteristic peak measurement; identifying a subset of the spectral pixels, at least one member of the subset being a pixel with a maximum spectral intensity, wherein the pixel with the maximum spectral intensity is a spectral pixel corresponding to a cell and having an associated characteristic peak measurement that is greater than or equal to a characteristic peak measurement associated with other spectral pixels corresponding to the cell; and forming a reconstructed cellular spectrum, the reconstructed cellular spectrum having a plurality of reconstructed measurements, each of the reconstructed measurements corresponding to a selected wavenumber, each of the reconstructed measurements being formed according to a sum of the intensity measurements associated with the selected wavenumber of the pixels in the subset.