Methods and kits for measuring von willebrand factor

摘要

Methods and kits for measuring levels of von Willebrand factor function in a sample without using a platelet aggregation agonist, such as ristocetin, comprising recombinant glycoprotein Ibα having at least two of a G233V, D235Y and M239V mutations and an agent to detect a complex between the recombinant glycoprotein Ibα and von Willebrand factor.

主权项

1. An isolated GPIbα polypeptide or functional fragment thereof, comprising an amino acid sequence that has at least 90% sequence identity to residues 1-290 of SEQ ID NO:11 and that includes at least two mutations relative to residues 1-290 of SEQ ID NO:11, wherein one of the mutations is D235Y;
wherein the isolated GPIbα polypeptide or fragment is capable of forming a complex with von Willebrand factor (VFW).