| 发明名称 | Method and composition for crystallizing G protein-coupled receptors | ||
| 摘要 | Certain embodiments provide a method for crystallizing a GPCR. The method may employ a fusion protein comprising: a) a first portion of a G-protein coupled receptor (GPCR), where the first portion comprises the TM1, TM2, TM3, TM4 and TM5 regions of the GPCR; b) a stable, folded protein insertion; and c) a second portion of the GPCR, where the second portion comprises the TM6 and TM7 regions of the GPCR. | ||
| 申请公布号 | US9045561(B2) | 申请公布日期 | 2015.06.02 |
| 申请号 | US201314133279 | 申请日期 | 2013.12.18 |
| 申请人 | THE BOARD OF TRUSTEE OF THE LELAND STANFORD JUNIOR UNIVERSITY | 发明人 | Kobilka Brian;Rosenbaum Daniel |
| 分类号 | C07K14/705;C07K14/72 | 主分类号 | C07K14/705 |
| 代理机构 | Bozicevic, Field & Francis LLP | 代理人 | Bozicevic, Field & Francis LLP ;Keddie James S. |
| 主权项 | 1. A fusion protein comprising, from N-terminus to C-terminus: a) a first portion of a G-protein coupled receptor (GPCR), wherein said first portion comprises TM1, TM2, TM3, TM4 and TM5 regions of said GPCR; b) a heterologous domain comprising an amino acid sequence that is at least 80% identical to the amino acid sequence of a soluble, autonomously folding, wild-type protein that aids in the formation of lattice contacts during crystallization of the fusion protein; c) a second portion of said GPCR, wherein said second portion comprises TM6 and TM7 regions of said GPCR. | ||
| 地址 | Palo Alto CA US | ||