Procedure for the purification of tiacumicin B

摘要

The present invention relates to an improved process for the purification of tiacumicin B. Specifically, the invention relates to a simplified, optimised process for the purification of tiacumicin B from a fermentation broth, using chromatography techniques. In particular, the invention relates to a method for purifying tiacumicin B which comprises subjecting a liquid containing tiacumicin B to at least one hydrophobic interaction chromatography step.;The process according to the invention is simpler than the processes according to the prior art, and can easily be used on a large scale for commercial production.

主权项

1. A process for purifying tiacumicin B comprising subjecting a liquid containing tiacumicin B to at least one hydrophobic interaction chromatography step, wherein the hydrophobic interaction chromatographic step comprises:
a) loading the liquid containing tiacumicin B at a pH ranging from 2.0 to 8.0 onto a hydrophobic interaction resin; b) eluting the impurities from the hydrophobic interaction resin with a mixture consisting of water and 10-52% acetonitrile at a pH at which the impurities are dissociated from the resin; c) eluting tiacumicin B from the hydrophobic interaction resin with a mixture of water and 48-60% acetonitrile at a pH at which the impurities are dissociated from the resin thereby obtaining purified tiacumicin B, wherein the liquid containing tiacumicin B is loaded twice onto the hydrophobic interaction resin, and wherein one hydrophobic interaction chromatography step uses, in elution steps b) and c), an eluent mixture having pH ranging from 2.0 to 5.0 and a subsequent hydrophobic interaction chromatography step uses, in elution steps b) and c), an eluent mixture having a pH range from 3.5 to 7.0, wherein the two chromatography steps are performed in any order.