发明名称 |
Method for producing immortalized memory b lymphocytes, immortalized b cell clone made by such method and monoclonal antibody obtained from an immortalized memory b lymphocyte or immortalized b cell clone for use in diagnosis or treatment of pathogenic or viral infection |
摘要 |
A method for producing a clone of an immortalised human B memory lymphocytes, comprising the step of transforming human B memory lymphocytes using Epstein Barr Virus (EBV) in the presence of a polyclonal B cell activator. The method is particularly useful in a method for producing a clone of an immortalised human B memory lymphocytes capable of producing a human monoclonal antibody with a desired antigen specificity, comprising the steps of : (i) selecting and isolating a human memory B lymphocyte subpopulation; (ii) transforming the subpopulation with Epstein Barr Virus (EBV) in the presence of a polyclonal B cell activator; (iii) screening the culture supernatant for antigen specificity; and (iv) isolating an immortalised human B memory lymphocyte clone capable of producing a human monoclonal antibody having the desired antigen specificity. |
申请公布号 |
IL183370(A) |
申请公布日期 |
2015.05.31 |
申请号 |
IL20070183370 |
申请日期 |
2007.05.21 |
申请人 |
INSTITUTE FOR RESEARCH IN BIOMEDICINE |
发明人 |
|
分类号 |
C07K;A61K39/395;A61P31/14;C07K16/00;C07K16/10;C07K16/12;C07K16/20;C12N5/0781;C12N5/24;C12P;C12P21/08;C12Q;G01N33/49 |
主分类号 |
C07K |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|