TWO-DIRECTIONAL SCANNING FOR LUMINESCENCE MICROSCOPY

摘要

In one form, a two-directional scanning method for luminescence microscopy is disclosed. A series of continuous scans are performed by an interrogation wide-field relative to a first direction and a target is identified. A precise position of the target is determined in the first direction. At least one scan by the interrogation wide-field is performed relative to a second direction at or near the precise position of the target in the first direction. The two-directional scanning method produces “on-the-fly” (i.e. ex tempore or impromptu) precise localization of targets. Embodiments open up new applications for background-free or background-reduced luminescence microscopy, for example time-gated or time-resolved luminescence microscopy, in a relatively fast, higher speed or more efficient manner.

主权项

1. A two-directional scanning method for luminescence microscopy, comprising:
performing a series of scans of a sample by an interrogation wide-field relative to a first direction and identifying a target; determining a precise position of the target in the first direction; and performing at least one scan by the interrogation wide-field, relative to a second direction, at or near the precise position of the target in the first direction.