METHOD OF IMPROVING THE SENSITIVITY OF COMPETITIVE IMMUNOASSAY

摘要

The present invention discloses a method improving the sensitivity of competitive immunoassay, mainly, the small molecular substances for detection first react with the antibody/receptor sufficiently, then react with the second molecules, which locates on the solid phase and participates the competitive reactions, in a short time, which may greatly improve the sensitivity of competitive immunoassay. The present invention owns an easy operation method and a low cost, does not require expensive equipments or professional technological personnel, also, it owns a high sensitivity and adapts to a plurality of immunoassay methods.

主权项

1. A method of improving the sensitivity of competitive immunoassay, wherein, the steps of the said method of improving the sensitivity of competitive immunoassay comprise:
pre-reaction: a substances for detection pre-react with a receptors/antibodies; re-reaction: a reaction products collected from the pre-reaction process react with a second molecules locating on a solid phase and attending the competitive reactions; during the said re-reaction process, the reaction product collected from the pre-reaction process is divided into one or more portions, then reacting with the said second molecules respectively.